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microcarrier cell culture technology  (Cytiva Europe)


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    Cytiva Europe microcarrier cell culture technology
    Effect of inoculum on viable cell density of Sertoli cells (SCs). Effect of inoculum on viable cell density for SCs grown in: (a) stirred <t>microcarrier</t> cultures and (b) control plates. Cells were plated at () 0.5 × 105, (•) 1 × 105 and () 2 × 105 cells/ml in 80 or 2.5 ml of DMEM, for the stirred and control cultures, respectively. For stirred cultures, 3 mg of Cytodex‐1 per mL was used. □, ○, △ stand for viabilities (%) of SCs, plated at () 0.5 × 105, (•) 1 × 105 and () 2 × 105 cells/ml in the figure. Media (50%) was changed every day from day 2.
    Microcarrier Cell Culture Technology, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 99/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microcarrier+cell+culture+technology/pmc06496594-330-16-20?v=Cytiva+Europe
    Average 99 stars, based on 94 article reviews
    microcarrier cell culture technology - by Bioz Stars, 2026-08
    99/100 stars

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    1) Product Images from "Expansion of mouse sertoli cells on microcarriers"

    Article Title: Expansion of mouse sertoli cells on microcarriers

    Journal: Cell Proliferation

    doi: 10.1111/j.1365-2184.2010.00677.x

    Effect of inoculum on viable cell density of Sertoli cells (SCs). Effect of inoculum on viable cell density for SCs grown in: (a) stirred microcarrier cultures and (b) control plates. Cells were plated at () 0.5 × 105, (•) 1 × 105 and () 2 × 105 cells/ml in 80 or 2.5 ml of DMEM, for the stirred and control cultures, respectively. For stirred cultures, 3 mg of Cytodex‐1 per mL was used. □, ○, △ stand for viabilities (%) of SCs, plated at () 0.5 × 105, (•) 1 × 105 and () 2 × 105 cells/ml in the figure. Media (50%) was changed every day from day 2.
    Figure Legend Snippet: Effect of inoculum on viable cell density of Sertoli cells (SCs). Effect of inoculum on viable cell density for SCs grown in: (a) stirred microcarrier cultures and (b) control plates. Cells were plated at () 0.5 × 105, (•) 1 × 105 and () 2 × 105 cells/ml in 80 or 2.5 ml of DMEM, for the stirred and control cultures, respectively. For stirred cultures, 3 mg of Cytodex‐1 per mL was used. □, ○, △ stand for viabilities (%) of SCs, plated at () 0.5 × 105, (•) 1 × 105 and () 2 × 105 cells/ml in the figure. Media (50%) was changed every day from day 2.

    Techniques Used: Control

    Initial and final cell densities, and fold increase of Sertoli cells grown in stirred microcarrier cultures and six‐well control plates
    Figure Legend Snippet: Initial and final cell densities, and fold increase of Sertoli cells grown in stirred microcarrier cultures and six‐well control plates

    Techniques Used: Control

    Available surface areas estimated in for stirred microcarrier bead cultures and control plates
    Figure Legend Snippet: Available surface areas estimated in for stirred microcarrier bead cultures and control plates

    Techniques Used: Control, Concentration Assay

    Apparent ( μ app ) and maximum ( μ max ) specific growth rates calculated using first order kinetic model for Sertoli cells (SCs) grown in stirred microcarrier cultures and control plates.
    Figure Legend Snippet: Apparent ( μ app ) and maximum ( μ max ) specific growth rates calculated using first order kinetic model for Sertoli cells (SCs) grown in stirred microcarrier cultures and control plates.

    Techniques Used: Control

    Microscopic observation of Sertoli cells grown at different microcarrier concentrations. Sertoli cells (SCs) plated at 1 × 105 cells/ml in 80 ml of DMEM with different microcarrier bead concentrations, observed on the 2nd day after inoculation.
    Figure Legend Snippet: Microscopic observation of Sertoli cells grown at different microcarrier concentrations. Sertoli cells (SCs) plated at 1 × 105 cells/ml in 80 ml of DMEM with different microcarrier bead concentrations, observed on the 2nd day after inoculation.

    Techniques Used:

    Substrate concentration and metabolite profiles during culture of Sertoli cells (SCs) on Cytodex‐1 microcarrier beads. Concentration (, •, , ) and specific production/consumption (□, ○, △, ▽) rates of glucose (a), lactate (b), glutamine (c) and ammonia (d) in culture represented. Values displayed represent average of three independent experiments. Error bars indicate the standard deviation of duplicate cultures. SCs (1 × 105 cells/ml) cultured with microcarrier bead concentration of 3 mg/ml. Media (50%) was changed every day, starting on day 2.
    Figure Legend Snippet: Substrate concentration and metabolite profiles during culture of Sertoli cells (SCs) on Cytodex‐1 microcarrier beads. Concentration (, •, , ) and specific production/consumption (□, ○, △, ▽) rates of glucose (a), lactate (b), glutamine (c) and ammonia (d) in culture represented. Values displayed represent average of three independent experiments. Error bars indicate the standard deviation of duplicate cultures. SCs (1 × 105 cells/ml) cultured with microcarrier bead concentration of 3 mg/ml. Media (50%) was changed every day, starting on day 2.

    Techniques Used: Concentration Assay, Standard Deviation, Cell Culture

    Optical and scanning electron micrographs of Sertoli cells (SCs) cultured on microcarrier beads under stirred culture conditions. SCs visualized using an optical microscope, day 2 (a), day 4 (b), day 6 (c) and day 8 (d) after MTT staining (200× amplification) for Cytodex‐1, respectively. SCs visualized using a scanning electron microscope, day 2 (e), day 4 (f), day 6 (g) and day 8 (h).
    Figure Legend Snippet: Optical and scanning electron micrographs of Sertoli cells (SCs) cultured on microcarrier beads under stirred culture conditions. SCs visualized using an optical microscope, day 2 (a), day 4 (b), day 6 (c) and day 8 (d) after MTT staining (200× amplification) for Cytodex‐1, respectively. SCs visualized using a scanning electron microscope, day 2 (e), day 4 (f), day 6 (g) and day 8 (h).

    Techniques Used: Cell Culture, Microscopy, Staining, Amplification

    Photomicrograph of neuronal stem cells (NSCs) cultured alone and NSCs co‐cultured with Sertoli cells (SCs). (a) NSCs cultured in six‐well chamber slides. (b) NSCs co‐cultured with SCs obtained from two‐dimensional culture. (c) NSCs co‐cultured with SCs obtained from microcarrier bead suspension culture. All photographed on day 3 after inoculation (the scale bar is 5 μm). Neurite outgrowth was marked with.
    Figure Legend Snippet: Photomicrograph of neuronal stem cells (NSCs) cultured alone and NSCs co‐cultured with Sertoli cells (SCs). (a) NSCs cultured in six‐well chamber slides. (b) NSCs co‐cultured with SCs obtained from two‐dimensional culture. (c) NSCs co‐cultured with SCs obtained from microcarrier bead suspension culture. All photographed on day 3 after inoculation (the scale bar is 5 μm). Neurite outgrowth was marked with.

    Techniques Used: Cell Culture, Suspension

    Nerve stem cells (NSCs) immunocytofluorescently labelled with anti‐nestin antibody to identify neurospheres (day 6). (a) NSCs cultured on six‐well chamber slides. (b) NSCs cultured with Sertoli cells (SCs) obtained from two‐dimensional culture. (c) NSCs cultured with SCs obtained from microcarrier bead culture.
    Figure Legend Snippet: Nerve stem cells (NSCs) immunocytofluorescently labelled with anti‐nestin antibody to identify neurospheres (day 6). (a) NSCs cultured on six‐well chamber slides. (b) NSCs cultured with Sertoli cells (SCs) obtained from two‐dimensional culture. (c) NSCs cultured with SCs obtained from microcarrier bead culture.

    Techniques Used: Cell Culture



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    microcarrier cell culture technology  (Cytiva Europe)
    99
    Cytiva Europe microcarrier cell culture technology
    Effect of inoculum on viable cell density of Sertoli cells (SCs). Effect of inoculum on viable cell density for SCs grown in: (a) stirred <t>microcarrier</t> cultures and (b) control plates. Cells were plated at () 0.5 × 105, (•) 1 × 105 and () 2 × 105 cells/ml in 80 or 2.5 ml of DMEM, for the stirred and control cultures, respectively. For stirred cultures, 3 mg of Cytodex‐1 per mL was used. □, ○, △ stand for viabilities (%) of SCs, plated at () 0.5 × 105, (•) 1 × 105 and () 2 × 105 cells/ml in the figure. Media (50%) was changed every day from day 2.
    Microcarrier Cell Culture Technology, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microcarrier+cell+culture+technology/pmc06496594-330-16-20?v=Cytiva+Europe
    Average 99 stars, based on 1 article reviews
    microcarrier cell culture technology - by Bioz Stars, 2026-08
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Effect of inoculum on viable cell density of Sertoli cells (SCs). Effect of inoculum on viable cell density for SCs grown in: (a) stirred microcarrier cultures and (b) control plates. Cells were plated at () 0.5 × 105, (•) 1 × 105 and () 2 × 105 cells/ml in 80 or 2.5 ml of DMEM, for the stirred and control cultures, respectively. For stirred cultures, 3 mg of Cytodex‐1 per mL was used. □, ○, △ stand for viabilities (%) of SCs, plated at () 0.5 × 105, (•) 1 × 105 and () 2 × 105 cells/ml in the figure. Media (50%) was changed every day from day 2.

    Journal: Cell Proliferation

    Article Title: Expansion of mouse sertoli cells on microcarriers

    doi: 10.1111/j.1365-2184.2010.00677.x

    Figure Lengend Snippet: Effect of inoculum on viable cell density of Sertoli cells (SCs). Effect of inoculum on viable cell density for SCs grown in: (a) stirred microcarrier cultures and (b) control plates. Cells were plated at () 0.5 × 105, (•) 1 × 105 and () 2 × 105 cells/ml in 80 or 2.5 ml of DMEM, for the stirred and control cultures, respectively. For stirred cultures, 3 mg of Cytodex‐1 per mL was used. □, ○, △ stand for viabilities (%) of SCs, plated at () 0.5 × 105, (•) 1 × 105 and () 2 × 105 cells/ml in the figure. Media (50%) was changed every day from day 2.

    Article Snippet: One gram dry weight of this support has an approximate surface area of 4400 cm 2 (Microcarrier Cell Culture Technology, GE Healthcare, Milwaukee, USA).

    Techniques: Control

    Initial and final cell densities, and fold increase of Sertoli cells grown in stirred microcarrier cultures and six‐well control plates

    Journal: Cell Proliferation

    Article Title: Expansion of mouse sertoli cells on microcarriers

    doi: 10.1111/j.1365-2184.2010.00677.x

    Figure Lengend Snippet: Initial and final cell densities, and fold increase of Sertoli cells grown in stirred microcarrier cultures and six‐well control plates

    Article Snippet: One gram dry weight of this support has an approximate surface area of 4400 cm 2 (Microcarrier Cell Culture Technology, GE Healthcare, Milwaukee, USA).

    Techniques: Control

    Available surface areas estimated in for stirred microcarrier bead cultures and control plates

    Journal: Cell Proliferation

    Article Title: Expansion of mouse sertoli cells on microcarriers

    doi: 10.1111/j.1365-2184.2010.00677.x

    Figure Lengend Snippet: Available surface areas estimated in for stirred microcarrier bead cultures and control plates

    Article Snippet: One gram dry weight of this support has an approximate surface area of 4400 cm 2 (Microcarrier Cell Culture Technology, GE Healthcare, Milwaukee, USA).

    Techniques: Control, Concentration Assay

    Apparent ( μ app ) and maximum ( μ max ) specific growth rates calculated using first order kinetic model for Sertoli cells (SCs) grown in stirred microcarrier cultures and control plates.

    Journal: Cell Proliferation

    Article Title: Expansion of mouse sertoli cells on microcarriers

    doi: 10.1111/j.1365-2184.2010.00677.x

    Figure Lengend Snippet: Apparent ( μ app ) and maximum ( μ max ) specific growth rates calculated using first order kinetic model for Sertoli cells (SCs) grown in stirred microcarrier cultures and control plates.

    Article Snippet: One gram dry weight of this support has an approximate surface area of 4400 cm 2 (Microcarrier Cell Culture Technology, GE Healthcare, Milwaukee, USA).

    Techniques: Control

    Microscopic observation of Sertoli cells grown at different microcarrier concentrations. Sertoli cells (SCs) plated at 1 × 105 cells/ml in 80 ml of DMEM with different microcarrier bead concentrations, observed on the 2nd day after inoculation.

    Journal: Cell Proliferation

    Article Title: Expansion of mouse sertoli cells on microcarriers

    doi: 10.1111/j.1365-2184.2010.00677.x

    Figure Lengend Snippet: Microscopic observation of Sertoli cells grown at different microcarrier concentrations. Sertoli cells (SCs) plated at 1 × 105 cells/ml in 80 ml of DMEM with different microcarrier bead concentrations, observed on the 2nd day after inoculation.

    Article Snippet: One gram dry weight of this support has an approximate surface area of 4400 cm 2 (Microcarrier Cell Culture Technology, GE Healthcare, Milwaukee, USA).

    Techniques:

    Substrate concentration and metabolite profiles during culture of Sertoli cells (SCs) on Cytodex‐1 microcarrier beads. Concentration (, •, , ) and specific production/consumption (□, ○, △, ▽) rates of glucose (a), lactate (b), glutamine (c) and ammonia (d) in culture represented. Values displayed represent average of three independent experiments. Error bars indicate the standard deviation of duplicate cultures. SCs (1 × 105 cells/ml) cultured with microcarrier bead concentration of 3 mg/ml. Media (50%) was changed every day, starting on day 2.

    Journal: Cell Proliferation

    Article Title: Expansion of mouse sertoli cells on microcarriers

    doi: 10.1111/j.1365-2184.2010.00677.x

    Figure Lengend Snippet: Substrate concentration and metabolite profiles during culture of Sertoli cells (SCs) on Cytodex‐1 microcarrier beads. Concentration (, •, , ) and specific production/consumption (□, ○, △, ▽) rates of glucose (a), lactate (b), glutamine (c) and ammonia (d) in culture represented. Values displayed represent average of three independent experiments. Error bars indicate the standard deviation of duplicate cultures. SCs (1 × 105 cells/ml) cultured with microcarrier bead concentration of 3 mg/ml. Media (50%) was changed every day, starting on day 2.

    Article Snippet: One gram dry weight of this support has an approximate surface area of 4400 cm 2 (Microcarrier Cell Culture Technology, GE Healthcare, Milwaukee, USA).

    Techniques: Concentration Assay, Standard Deviation, Cell Culture

    Optical and scanning electron micrographs of Sertoli cells (SCs) cultured on microcarrier beads under stirred culture conditions. SCs visualized using an optical microscope, day 2 (a), day 4 (b), day 6 (c) and day 8 (d) after MTT staining (200× amplification) for Cytodex‐1, respectively. SCs visualized using a scanning electron microscope, day 2 (e), day 4 (f), day 6 (g) and day 8 (h).

    Journal: Cell Proliferation

    Article Title: Expansion of mouse sertoli cells on microcarriers

    doi: 10.1111/j.1365-2184.2010.00677.x

    Figure Lengend Snippet: Optical and scanning electron micrographs of Sertoli cells (SCs) cultured on microcarrier beads under stirred culture conditions. SCs visualized using an optical microscope, day 2 (a), day 4 (b), day 6 (c) and day 8 (d) after MTT staining (200× amplification) for Cytodex‐1, respectively. SCs visualized using a scanning electron microscope, day 2 (e), day 4 (f), day 6 (g) and day 8 (h).

    Article Snippet: One gram dry weight of this support has an approximate surface area of 4400 cm 2 (Microcarrier Cell Culture Technology, GE Healthcare, Milwaukee, USA).

    Techniques: Cell Culture, Microscopy, Staining, Amplification

    Photomicrograph of neuronal stem cells (NSCs) cultured alone and NSCs co‐cultured with Sertoli cells (SCs). (a) NSCs cultured in six‐well chamber slides. (b) NSCs co‐cultured with SCs obtained from two‐dimensional culture. (c) NSCs co‐cultured with SCs obtained from microcarrier bead suspension culture. All photographed on day 3 after inoculation (the scale bar is 5 μm). Neurite outgrowth was marked with.

    Journal: Cell Proliferation

    Article Title: Expansion of mouse sertoli cells on microcarriers

    doi: 10.1111/j.1365-2184.2010.00677.x

    Figure Lengend Snippet: Photomicrograph of neuronal stem cells (NSCs) cultured alone and NSCs co‐cultured with Sertoli cells (SCs). (a) NSCs cultured in six‐well chamber slides. (b) NSCs co‐cultured with SCs obtained from two‐dimensional culture. (c) NSCs co‐cultured with SCs obtained from microcarrier bead suspension culture. All photographed on day 3 after inoculation (the scale bar is 5 μm). Neurite outgrowth was marked with.

    Article Snippet: One gram dry weight of this support has an approximate surface area of 4400 cm 2 (Microcarrier Cell Culture Technology, GE Healthcare, Milwaukee, USA).

    Techniques: Cell Culture, Suspension

    Nerve stem cells (NSCs) immunocytofluorescently labelled with anti‐nestin antibody to identify neurospheres (day 6). (a) NSCs cultured on six‐well chamber slides. (b) NSCs cultured with Sertoli cells (SCs) obtained from two‐dimensional culture. (c) NSCs cultured with SCs obtained from microcarrier bead culture.

    Journal: Cell Proliferation

    Article Title: Expansion of mouse sertoli cells on microcarriers

    doi: 10.1111/j.1365-2184.2010.00677.x

    Figure Lengend Snippet: Nerve stem cells (NSCs) immunocytofluorescently labelled with anti‐nestin antibody to identify neurospheres (day 6). (a) NSCs cultured on six‐well chamber slides. (b) NSCs cultured with Sertoli cells (SCs) obtained from two‐dimensional culture. (c) NSCs cultured with SCs obtained from microcarrier bead culture.

    Article Snippet: One gram dry weight of this support has an approximate surface area of 4400 cm 2 (Microcarrier Cell Culture Technology, GE Healthcare, Milwaukee, USA).

    Techniques: Cell Culture